Chapter 14. Protein and Lipid Partitioning in Locally Heterogeneous Model Membranes

  1. Prof. Dr. Lukas K. Tamm
  1. Prof. Petra Schwille,
  2. Prof. Nicoletta Kahya,
  3. Kirsten Bacia

Published Online: 29 MAR 2006

DOI: 10.1002/3527606769.ch14

Book Title

Protein-Lipid Interactions: From Membrane Domains to Cellular Networks

Protein-Lipid Interactions: From Membrane Domains to Cellular Networks

Additional Information

How to Cite

Schwille, P., Kahya, N. and Bacia, K. (2006) Protein and Lipid Partitioning in Locally Heterogeneous Model Membranes, in Protein-Lipid Interactions: From Membrane Domains to Cellular Networks (ed L. K. Tamm), Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, FRG. doi: 10.1002/3527606769.ch14

Editor Information

  1. Professor of Molecular Physiology and Biological Physics, University of Virginia, PO Box 800736, Charlottesville, VA 22908-0736, USA

Author Information

  1. Biophysics/BioTec Technical University Dresden, Tatzberg 47–51, 01307 Dresden, Germany

Publication History

  1. Published Online: 29 MAR 2006
  2. Published Print: 5 AUG 2005

ISBN Information

Print ISBN: 9783527311514

Online ISBN: 9783527606764

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Keywords:

  • protein and lipid partioning in locally heterogeneous model membranes;
  • complex membrane organization;
  • biomimetic membranes;
  • investigation of domain formation in biomimetic membranes;
  • lipid domain assembly in GUVs;
  • spatial organization and dynamics of membrane proteins in GUVs

Summary

This chapter contains sections titled:

  • Introduction: Why Should We Use Simple Model Membranes to Gain Insight into Complex Membrane Organization?

    • Relation of Domain Structure to a Biological Function

    • An Accessible Detection Method

    • The Term “Raft”

  • Biomimetic Membranes

    • GUVs: Properties and Preparation

  • Methods of Investigation of Domain Formation in Biomimetic Membranes

    • Electron Microscopy

    • Atomic Force Microscopy (AFM)

    • Near-field Scanning Optical Microscopy (NSOM)

    • Fluorescence Imaging (Confocal, Multi-photon)

    • Fluorescence Photobleaching Recovery (FPR) or Fluorescence Recovery after Photobleaching (FRAP)

    • Single Particle Tracking (SPT)

    • Fluorescence Correlation Spectroscopy (FCS)

  • Lipid Domain Assembly in GUVs

    • Phase Separation

      • Can Cellular Membrane Domains be Regarded as Phase Domains?

      • Properties of Lipid Bilayer Phases

      • Co-existence of Lipid Bilayer Phases

      • Lipid Phase Diagrams

    • Binary Lipid Systems

    • Ternary Lipid Systems

    • Effect of Sterols on Lipid Segregation

    • Lipid Dynamics in Domain-exhibiting GUVs

      • “Fluidizing” Effect of Cholesterol for High-Tm Lipids

      • “Condensing” Effect of Cholesterol for Low-Tm Lipids

  • Spatial Organization and Dynamics of Membrane Proteins in GUVs

  • From Model to Cellular Membranes

    • Model Membranes Constitute Test Systems for Developing New and Improving Existing Detection Techniques

    • Direct Comparison Between Results Obtained on Model and Native Membranes

    • Model Membranes Demonstrate What Structures Can be Potentially Formed by Lipids and Proteins, and Suggest Mechanisms for Fulfilling in vivo Functions

  • References

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