Chapter 16. Structure and Interactions of C2 Domains at Membrane Surfaces

  1. Prof. Dr. Lukas K. Tamm
  1. Prof. David S. Cafiso

Published Online: 29 MAR 2006

DOI: 10.1002/3527606769.ch16

Protein-Lipid Interactions: From Membrane Domains to Cellular Networks

Protein-Lipid Interactions: From Membrane Domains to Cellular Networks

How to Cite

Cafiso, D. S. (2006) Structure and Interactions of C2 Domains at Membrane Surfaces, in Protein-Lipid Interactions: From Membrane Domains to Cellular Networks (ed L. K. Tamm), Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, FRG. doi: 10.1002/3527606769.ch16

Editor Information

  1. Professor of Molecular Physiology and Biological Physics, University of Virginia, PO Box 800736, Charlottesville, VA 22908-0736, USA

Author Information

  1. Department of Chemistry, University of Virginia, McCormick Road, Charlottesville, VA 22908-4319, USA

Publication History

  1. Published Online: 29 MAR 2006
  2. Published Print: 5 AUG 2005

ISBN Information

Print ISBN: 9783527311514

Online ISBN: 9783527606764

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Keywords:

  • structure and interactions of C2 domains at membrane surfaces;
  • Ca2+-dependent and Ca2+-independent membrane binding;
  • electrostatic binding of simple linear protein motifs;
  • electrostatic calculations on C2 domains;
  • proteins with multiple C2 domains;
  • interactions of phosphoinositides with C2 domains

Summary

This chapter contains sections titled:

  • Introduction

  • C2 Domains: Ca2+-dependent and Ca2+-independent Membrane Binding

  • What Drives Membrane Targeting of C2 Domains?

  • Electrostatic Binding of Simple Linear Protein Motifs

  • The Results of Electrostatic Calculations on C2 Domains

  • Determining the Interactions and Positions of C2 Domains

    • Site-directed Mutagenesis

    • Chemical Labeling

    • Fluorescence

    • Site-directed Spin Labeling (SDSL) to Determine C2 Domain Orientation

  • Proteins with Multiple C2 Domains

  • Interactions of Phosphoinositides with C2 Domains

  • References