Chapter 11. Adhesion Molecule-Modified Cardiovascular Prostheses: Characterization of Cellular Adhesion in a Cell Culture Model and by Cellular Force Spectroscopy

  1. Prof. Dr. Wulff Possart
  1. U. Bakowsky1,
  2. C. Ehrhardt2,
  3. C. Loehbach2,
  4. P. Li2,
  5. C. Kneuer3,
  6. D. Jahn4,
  7. D. Hoekstra5 and
  8. C.-M. Lehr2

Published Online: 28 APR 2006

DOI: 10.1002/3527607307.ch11

Adhesion: Current Research and Applications

Adhesion: Current Research and Applications

How to Cite

Bakowsky, U., Ehrhardt, C., Loehbach, C., Li, P., Kneuer, C., Jahn, D., Hoekstra, D. and Lehr, C.-M. (2005) Adhesion Molecule-Modified Cardiovascular Prostheses: Characterization of Cellular Adhesion in a Cell Culture Model and by Cellular Force Spectroscopy, in Adhesion: Current Research and Applications (ed W. Possart), Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, FRG. doi: 10.1002/3527607307.ch11

Editor Information

  1. Laboratoire Européen de Recherche Universitaire Saarland-Lorraine (LERUSL), Universität des Saarlandes, Gebäude 22-6, Postfach 151150, 66041 Saarbrücken, Germany

Author Information

  1. 1

    Department of Pharmaceutical Technology and Biopharmaceutics, Philipps University Marburg, Postfach, 35032 Marburg, Germany

  2. 2

    Department of Biopharmaceutics and Pharmaceutical Technology, Saarland University, 66123 Saarbrücken, Germany

  3. 3

    Leipzig University, Department of Pharmacology, Pharmacy and Toxicology, An den Tierkliniken 15, 04103 Leipzig, Germany

  4. 4

    Institute of Microbiology, Technical University of Braunschweig, 38106 Braunschweig, Germany

  5. 5

    Department of Membrane Cell Biology, University of Groningen, 9700 AV Groningen, Netherlands

Publication History

  1. Published Online: 28 APR 2006
  2. Published Print: 24 AUG 2005

ISBN Information

Print ISBN: 9783527312634

Online ISBN: 9783527607303

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Keywords:

  • adhesion;
  • research;
  • application;
  • adhesion molecule-modified cardiovascular prostheses;
  • characterization of cellular adhesion;
  • cell culture model;
  • cellular force spectroscopy;
  • cell adhesion force measurements

Summary

This chapter contains sections titled:

  • Abstract

  • Introduction

  • Materials and Methods

    • Chemicals for the Modification

    • Implant Materials

    • Modification of the PTFE Surface

    • Scanning Force Microscopy

    • Fourier Transform Infrared Spectroscopy

    • Environmental Scanning Electron Microscopy

    • Confocal Laser Scanning Microscopy (CLSM)

    • Isolation and Culture of HUVECs

    • Endothelialization of PTFE Films

  • Results and Discussion

    • Wet-Chemical Modification of PTFE Polymer Film

    • Cell Adhesion Experiments

      • Adhesion and Cultivation in Static Culture

      • Perfusion Experiments

    • Cell Adhesion Force Measurements

  • Conclusion

  • Acknowledgments

  • References