Chapter 18. Autoantibodies and the Cloning and Characterization of Cellular Constituents†

  1. Prof. Dr. K. Michael Pollard
  1. Edward K. L. Chan

Published Online: 31 MAY 2006

DOI: 10.1002/3527607854.ch18

Book Title

Autoantibodies and Autoimmunity: Molecular Mechanisms in Health and Disease

Autoantibodies and Autoimmunity: Molecular Mechanisms in Health and Disease

Additional Information

How to Cite

Chan, E. K. L. (2006) Autoantibodies and the Cloning and Characterization of Cellular Constituents, in Autoantibodies and Autoimmunity: Molecular Mechanisms in Health and Disease (ed K. M. Pollard), Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, FRG. doi: 10.1002/3527607854.ch18

Editor Information

  1. Department of Molecular and Experimental Medicine, The Scripps Research Institute, MEM 131, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA

Author Information

  1. Department of Oral Biology, University of Florida, P.O. Box 100424, Gainesville, FL 32610-0424, USA

  1. †

    A list of abbreviations used is located at the end of this chapter.

Publication History

  1. Published Online: 31 MAY 2006
  2. Published Print: 25 NOV 2005

ISBN Information

Print ISBN: 9783527311415

Online ISBN: 9783527607853

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Keywords:

  • autoantibodies;
  • cloning and characterization of cellular constituents;
  • cDNA expression cloning as a tool to identify autoantigens;
  • autoantibodies to IGF-II mRNA-binding protein p62 and overexpression of p62 in human hepatocellular carcinoma;
  • Golgi complex autoantigens;
  • cloning of GW182 autoantigen and identification of GW bodies

Summary

This chapter contains sections titled:

  • Introduction

  • cDNA Expression Cloning as a Tool to Identify Autoantigens

    • Selection of Screening Sera Is Critical to Successful Cloning

    • Selection of cDNA Libraries Is Another Critical Factor of Successful Cloning

    • Useful Controls in Expression Cloning

    • Methods in Verification of Candidates in Expression Cloning

    • Further Characterization of cDNA Clones

  • Autoantibodies to IGF-II mRNA-binding Protein p62 and Overexpression of p62 in Human Hepatocellular Carcinoma

    • Cloning of IGF-II mRNA-binding Autoantigen p62 in HCC

    • Humoral Response to p62 During Transition from Chronic Liver Disease to HCC

    • Autoantibodies to p62 and Koc Are Widely Present in HCC and Other Cancers

    • Aberrant Expression of p62 in HCC

  • Unique Features of Golgi Complex Autoantigens

    • Cloning of Golgi Autoantigens (Golgins)

    • Prevalence of Human AGA

    • Mechanism of AGA Production and Relation to Other Large Coiled-coil Protein Autoantigens

    • Modification of Golgi Autoantigens During Cell Death

  • Cloning of GW182 Autoantigen and Identification of GW Bodies

    • GW Bodies Are Distinct Cytoplasmic Foci

    • GWBs Are Foci for mRNA Storage and Degradation

    • GW182 and Sjögrens Syndrome

  • Future Perspectives

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