Chapter 3. The Biophysical Characterization of Lipid Rafts

  1. Prof. Dr. Christopher J. Fielding4,5
  1. Pranav Sharma1,
  2. Rajat Varma2,
  3. Satyajit Mayor3

Published Online: 6 JUN 2006

DOI: 10.1002/3527608079.ch3

Book Title

Lipid Rafts and Caveolae: From Membrane Biophysics to Cell Biology

Lipid Rafts and Caveolae: From Membrane Biophysics to Cell Biology

Additional Information

How to Cite

Sharma, P., Varma, R. and Mayor, S. (2006) The Biophysical Characterization of Lipid Rafts, in Lipid Rafts and Caveolae: From Membrane Biophysics to Cell Biology (ed C. J. Fielding), Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, FRG. doi: 10.1002/3527608079.ch3

Editor Information

  1. 4

    Cardiovascular Research Institute and Department of Medicine, University of California San Francisco, San Francisco, CA 94143, USA

  2. 5

    Cardiovascular Research Institute and Department of Physiology, University of California San Francisco, San Francisco, CA 94143, USA

Author Information

  1. 1

    Cold Spring Harbor Laboratories, Cold Spring Harbor, New York 11724, USA

  2. 2

    Program in Molecular Pathogenesis, Skirball Institute for Biomolecular Medicine, New York University School of Medicine, 540 First Avenue, New York 10016, USA

  3. 3

    National Centre for Biological Sciences, UAS-GKVK Campus, Bangalore 560065, India

Publication History

  1. Published Online: 6 JUN 2006
  2. Published Print: 9 JAN 2006

ISBN Information

Print ISBN: 9783527312610

Online ISBN: 9783527608072

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CHAPTER TOOLS

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Keywords:

  • lipid rafts;
  • caveolae;
  • biophysical characterization of lipid rafts;
  • origin of the raft hypothesis;
  • role of lipid-anchored proteins in the development of the membrane raft hypothesis;
  • diffusion-based measurements;
  • proximity measurements

Summary

This chapter contains sections titled:

  • Introduction: The Fluid Mosaic Model and Membrane Domains

  • The Origin of the Raft Hypothesis

  • The Role of Lipid-Anchored Proteins in the Development of the Membrane Raft Hypothesis

  • The Case For and Against DRMs as Evidence for “Rafts” in Cell Membranes

  • Why Are Biophysical Studies Useful for Understanding Lipid Rafts?

  • Diffusion-Based Measurements

    • Single-Molecule Studies

    • Fluorescence Recovery After Photobleaching

    • Fluorescence Correlation Spectroscopy

  • Proximity Measurements

    • Proximity Measurement Using Homo-FRET

    • Proximity Measurement Using Hetero-FRET

  • Conclusions

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