Chapter 8. Microarray Biochips – Thousands of Reactions on a Small Chip (MOBA)

  1. Prof. Dr. Jürgen Popp10,11 and
  2. Dr. Marion Strehle10
  1. Wolfgang Mönch1,†,
  2. Johannes Donauer2,
  3. Bernd M. Fischer3,
  4. Rüdiger Frank4,
  5. Günter Gauglitz4,
  6. Carsten Glasenapp1,
  7. Hanspeter Helm5,
  8. Paul Hing6,
  9. Matthias Hoffmann5,
  10. Peter Uhd Jepsen7,
  11. Thomas Kleine-Ostmann8,
  12. Martin Koch8,
  13. Holger Krause1,
  14. Nicolae Leopold4,
  15. Tina Mutschler4,
  16. Frank Rutz8,
  17. Titus Sparna9 and
  18. Hans Zappe1

Published Online: 4 AUG 2006

DOI: 10.1002/3527608842.ch8

Biophotonics: Visions for Better Health Care

Biophotonics: Visions for Better Health Care

How to Cite

Mönch, W., Donauer, J., Fischer, B. M., Frank, R., Gauglitz, G., Glasenapp, C., Helm, H., Hing, P., Hoffmann, M., Jepsen, P. U., Kleine-Ostmann, T., Koch, M., Krause, H., Leopold, N., Mutschler, T., Rutz, F., Sparna, T. and Zappe, H. (2006) Microarray Biochips – Thousands of Reactions on a Small Chip (MOBA), in Biophotonics: Visions for Better Health Care (eds J. Popp and M. Strehle), Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, FRG. doi: 10.1002/3527608842.ch8

Editor Information

  1. 10

    Institut für Physikalische Chemie, Friedrich-Schiller-Universität Jena, Helmholtzweg 4, 07743 Jena, Germany

  2. 11

    Institut für Physikalische Hochtechnologie, Albert-Einstein-Str. 9, 07745 Jena, Germany

Author Information

  1. 1

    Laboratory for Micro-optics, Department of Microsystems Engineering – IMTEK, University of Freiburg, Georges-Koehler-Allee 102, 79110 Freiburg, Germany

  2. 2

    University Hospital Freiburg, Hugstetter Straße 55, 79106 Freiburg, Germany

  3. 3

    Freiburger Materialforschungszentrum, Stefan-Meier-Straße 21, 79104 Freiburg, Germany

  4. 4

    Eberhard-Karls University, Institute of Physical and Theoretical Chemistry, Auf der Morgenstelle 8, 72076 Tübingen, Germany

  5. 5

    Department of Molecular and Optical Physics, University of Freiburg, Stefan-Meier-Str. 19, 79104 Freiburg, Germany

  6. 6

    Sensovation AG, Ludwigshafener Straße 29, 78333 Stockach, Germany

  7. 7

    Research Center COM, Technical University of Denmark, 2800 Kgs. Lyngby, Denmark

  8. 8

    Institut für Hochfrequenztechnik, TU Braunschweig, Schleinitzstraße 22, 38106 Braunschweig, Germany

  9. 9

    University Hospital Freiburg, Core Facility Genomics, Stefan Meier Straße 19, 79106 Freiburg, Germany

  1. Corresponding author; project: MOBA – Micro-Optical Biochip Analysis

Publication History

  1. Published Online: 4 AUG 2006
  2. Published Print: 22 MAY 2006

ISBN Information

Print ISBN: 9783527406227

Online ISBN: 9783527608843

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Keywords:

  • biophotonics;
  • better health care;
  • microarray biochips;
  • fluorescence techniques;
  • optical systems for fluorescence analysis;
  • label-free techniques;
  • terahertz spectroscopy

Summary

This chapter contains sections titled:

  • Introduction

  • Microarrays: Biological Background

    • Principle of the Microarray Experiment

    • Oligonucleotide Microarrays

    • cDNA Arrays

    • Production of cDNA Microarrays

    • Hybridization to cDNA Microarrays

    • Analysis of Data Generated from Microarray Experiments

      • Low-level Data Analysis

      • High-level Analysis

    • Application of Microarrays in Medical Research

      • Deciphering of New Gene Regulations

      • Time-series Gene Regulation Data

      • Diagnostics

      • Pharmacogenomics

  • Fluorescence Techniques

    • CCD Sensors in Fluorescence Analysis: State-of-the-Art

  • Optical Systems for Fluorescence Analysis

    • Biochip Readers: State-of-the-Art

    • New Developments

      • Dynamic Holographic Excitation of Microarray Biochips

      • Diffractive Optical Elements: Fundamental Considerations

      • Optimization of Holographic Excitation Using DDOEs

    • Spectral Fluorescence Detection

      • Instrumentation

      • Quantitative Detection of Concentrations

      • Detection of Emission Spectra

    • Advantages of Dynamic Holographic Excitation of Microarray Biochips

  • Label-free Techniques

    • Surface Chemistry

    • Surface Characterization

    • Reflectometric Interference Spectroscopy (RIfS) Sensing

    • Surface Plasmon Resonance (SPR) and Mach–Zehnder Techniques

  • Terahertz Spectroscopy

    • Principles of THz Spectroscopy

      • THz Time Domain Spectroscopy

      • Photomixing

    • Experimental Advances

      • Femtosecond Optical Pulses Guided by Optical Fibers

      • Spiral Optical Delay Line

      • Stabilization of the Two-color Lasers

    • On-chip Techniques

    • Design and Fabrication of Efficient Filters

    • THz Spectroscopy on Biomolecules

    • THz Spectroscopy of Spotted RNA

    • THz Spectroscopy: A Technology with High Future Potential

  • Outlook

  • Glossary

  • Key References

  • References