Standard Article

Proteins: Postsynthetic Modification – Function and Physical Analysis

  1. Brigitte Wittmann-Liebold1,
  2. Theodora Choli-Papadopoulou2

Published Online: 28 SEP 2007

DOI: 10.1002/9780470015902.a0000551.pub2



How to Cite

Wittmann-Liebold, B. and Choli-Papadopoulou, T. 2007. Proteins: Postsynthetic Modification – Function and Physical Analysis. eLS. .

Author Information

  1. 1

    Wittmann Institute of Technology and Analysis, WITA GmbH, Germany

  2. 2

    Aristotle University of Thessaloniki, School of Chemistry, Greece

Publication History

  1. Published Online: 28 SEP 2007


Analysis of the entire genome (genomics) provides static information about the genes of an organism. In contrast, total protein analysis (proteomics) reflects the dynamic situation, providing information about the rate of expression and regulation of individual proteins within the cell. Clues as to the function of proteins in diverse cell processes can only be obtained if their structure and modifications or variations are fully understood. Recently, we learned to define important individual posttranslational protein modifications for function.


  • protein modifications;
  • structure and function of modifications;
  • identification of modifications;
  • 2DE separation and MS analysis;
  • protein processing;
  • phosphorylation