Standard Article

Restriction Enzymes

  1. Mala Mani1,
  2. Karthikeyan Kandavelou2,
  3. Srinivasan Chandrasegaran2

Published Online: 21 DEC 2007

DOI: 10.1002/9780470015902.a0000973.pub3

eLS

eLS

How to Cite

Mani, M., Kandavelou, K. and Chandrasegaran, S. 2007. Restriction Enzymes. eLS. .

Author Information

  1. 1

    Dana Farber Cancer Institute, Boston, Massachusetts, USA

  2. 2

    Johns Hopkins University, Baltimore, Maryland, USA

Publication History

  1. Published Online: 21 DEC 2007

Abstract

Type II restriction enzymes are the molecular scissors that catalyse the double-strand cleavage of deoxyribonucleic acid (DNA) at specific base sequences. They are essential tools for manipulating DNA including but not limited to cloning, analysis and sequencing. Recent advances have made it possible to design and engineer chimaeric nucleases to target specific DNA sites within a genome thus making them useful tools to carry out gene therapy.

Keywords:

  • restriction enzymes;
  • endonucleases;
  • methylases;
  • restriction–modification;
  • recombinant DNA;
  • chimaeric nucleases