Published Online: 21 DEC 2007
Copyright © 2001 John Wiley & Sons, Ltd. All rights reserved.
How to Cite
Mani, M., Kandavelou, K. and Chandrasegaran, S. 2007. Restriction Enzymes. eLS. .
- Published Online: 21 DEC 2007
Type II restriction enzymes are the molecular scissors that catalyse the double-strand cleavage of deoxyribonucleic acid (DNA) at specific base sequences. They are essential tools for manipulating DNA including but not limited to cloning, analysis and sequencing. Recent advances have made it possible to design and engineer chimaeric nucleases to target specific DNA sites within a genome thus making them useful tools to carry out gene therapy.
- restriction enzymes;
- recombinant DNA;
- chimaeric nucleases