Gene Synthesis for Protein Production
Published Online: 28 SEP 2007
Copyright © 2001 John Wiley & Sons, Ltd. All rights reserved.
How to Cite
Stewart, L. 2007. Gene Synthesis for Protein Production. eLS. .
- Published Online: 28 SEP 2007
Synthetic gene production is an enabling technology for improved protein production since it can be used to produce totally novel gene sequences that are optimized for codon usage and other sequence features anticipated to facilitate improved protein expression in defined expression systems. Computer aided gene design algorithms of increasing sophistication, allow researchers to exploit the degeneracy of the genetic code to engineer expression optimized synthetic gene sequences and the overlapping oligonucleotides needed to manufacture the desired synthetic gene by polymerase chain reaction (PCR) methods. Low cost synthetic gene production is ushering in a new era of synthetic biology for improved protein production.
- gene synthesis;
- codon usage;
- genetic code;
- protein production;
- gene design