Standard Article

Gene Synthesis for Protein Production

  1. Lance Stewart

Published Online: 28 SEP 2007

DOI: 10.1002/9780470015902.a0020211

eLS

eLS

How to Cite

Stewart, L. 2007. Gene Synthesis for Protein Production. eLS. .

Author Information

  1. deCODE biostructures, Inc., Washington, USA

Publication History

  1. Published Online: 28 SEP 2007

Abstract

Synthetic gene production is an enabling technology for improved protein production since it can be used to produce totally novel gene sequences that are optimized for codon usage and other sequence features anticipated to facilitate improved protein expression in defined expression systems. Computer aided gene design algorithms of increasing sophistication, allow researchers to exploit the degeneracy of the genetic code to engineer expression optimized synthetic gene sequences and the overlapping oligonucleotides needed to manufacture the desired synthetic gene by polymerase chain reaction (PCR) methods. Low cost synthetic gene production is ushering in a new era of synthetic biology for improved protein production.

Keywords:

  • gene synthesis;
  • codon usage;
  • genetic code;
  • protein production;
  • gene design