Tandem Affinity Purification (TAP) Tags
Published Online: 28 SEP 2007
Copyright © 2001 John Wiley & Sons, Ltd. All rights reserved.
How to Cite
Damasceno, C. M. B. and Rose, J. K. C. 2007. Tandem Affinity Purification (TAP) Tags. eLS. .
- Published Online: 28 SEP 2007
This is not the most recent version of the article. View current version (15 AUG 2014)
Tandem affinity purification (TAP) tagging is a method to purify multimeric protein complexes that can be used under essentially physiological conditions. This technique allows subsequent protein identification by mass spectrometry or functional analysis of the proteins post-purification. The TAP method has already been used to help elucidate large networks of interacting proteins in several organisms.
- tandem affinity purification;
- protein-protein interaction;
- protein network;
- protein purification