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Tandem Affinity Purification (TAP) Tags

  1. Cynthia Maria Borges Damasceno,
  2. Jocelyn Kenneth Campbell Rose

Published Online: 28 SEP 2007

DOI: 10.1002/9780470015902.a0020212

eLS

eLS

How to Cite

Damasceno, C. M. B. and Rose, J. K. C. 2007. Tandem Affinity Purification (TAP) Tags. eLS. .

Author Information

  1. Cornell University, Ithaca, New York, USA

Publication History

  1. Published Online: 28 SEP 2007

This is not the most recent version of the article. View current version (15 AUG 2014)

Abstract

Tandem affinity purification (TAP) tagging is a method to purify multimeric protein complexes that can be used under essentially physiological conditions. This technique allows subsequent protein identification by mass spectrometry or functional analysis of the proteins post-purification. The TAP method has already been used to help elucidate large networks of interacting proteins in several organisms.

Keywords:

  • tandem affinity purification;
  • protein-protein interaction;
  • protein network;
  • protein purification