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Gene Inactivation and Tissue-specific Metabolism

  1. Matthew T Flowers,
  2. James M Ntambi

Published Online: 30 APR 2008

DOI: 10.1002/9780470015902.a0021020



How to Cite

Flowers, M. T. and Ntambi, J. M. 2008. Gene Inactivation and Tissue-specific Metabolism. eLS. .

Author Information

  1. University of Wisconsin-Madison, Madison, Wisconsin, USA

Publication History

  1. Published Online: 30 APR 2008

This is not the most recent version of the article. View current version (15 OCT 2014)


The application of gene inactivation strategies targeting genes with various metabolic functions has greatly advanced our understanding of metabolism at both the cellular and physiological level. Whole-body genomic ablation of a gene of interest may be achieved using transgenic methods to introduce a null allele. Alternatively, the Cre-loxP recombinase system allows for the generation of animals harbouring a tissue-specific gene inactivation. Another approach is to alter the messenger ribonucleic acid (mRNA) abundance of the target gene using RNA interference (RNAi) or antisense oligonucleotide (ASO) methods. Together, these strategies help elucidate gene function through association of metabolic phenotypes with gene inactivation at a defined genetic locus.


  • transgenic mice;
  • metabolism;
  • gene knockout;
  • RNA interference;
  • Cre-loxP