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Biomolecular NMR Spectroscopy of Ribonucleic Acids

  1. Thorsten Dieckmann1,
  2. Pascale Legault2

Published Online: 30 APR 2008

DOI: 10.1002/9780470015902.a0021033

eLS

eLS

How to Cite

Dieckmann, T. and Legault, P. 2008. Biomolecular NMR Spectroscopy of Ribonucleic Acids. eLS. .

Author Information

  1. 1

    University of Waterloo, Waterloo, Ontario, Canada

  2. 2

    Université de Montréal, Montréal, Quebec, Canada

Publication History

  1. Published Online: 30 APR 2008

This is not the most recent version of the article. View current version (15 JUL 2014)

Abstract

Biomolecular nuclear magnetic resonance (NMR) spectroscopy allows the characterization of structural and dynamic properties of ribonucleic acids (RNAs) in solution. The NMR-based determination of high-resolution three-dimensional (3D) structures has been achieved for RNAs up to ca. 100 nucleotides. Biomolecular NMR also provides valuable information about RNA–ligand interactions and the role of metal ions and shifted pKa in RNA structure and catalysis.

Keywords:

  • RNA structure;
  • isotope labelling;
  • molecular dynamics;
  • metal ion binding