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Factor-Mediated Ribosome Assembly in Bacteria

  1. A Dönhöfer1,
  2. MR Sharma2,
  3. PP Datta2,
  4. KH Nierhaus3,
  5. RK Agrawal2,
  6. DN Wilson1

Published Online: 15 SEP 2009

DOI: 10.1002/9780470015902.a0021836

eLS

eLS

How to Cite

Dönhöfer, A., Sharma, M., Datta, P., Nierhaus, K., Agrawal, R. and Wilson, D. 2009. Factor-Mediated Ribosome Assembly in Bacteria. eLS. .

Author Information

  1. 1

    University of Munich, Gene Center and Department of Chemistry and Biochemistry, Germany

  2. 2

    Wadsworth Center, New York State Department of Health, Albany, New York, USA

  3. 3

    Max-Planck-Institute for Molecular Genetics, Berlin, Germany

Publication History

  1. Published Online: 15 SEP 2009

Abstract

The ribosome is a structurally and functionally conserved macromolecular machine responsible for translating messenger ribonucleic acid (mRNA) into proteins. Composed of two independently assembled subunits, the bacterial ribosome is approximately 2.5 MDa in size. The process of translation, catalysed by the ribosome, is central to gene expression in the cell. Correspondingly, the regulation of ribosome biogenesis is paramount to cell viability, growth and differentiation. Moreover, the ribosome is the target of numerous clinically relevant therapeutic agents, several of which are known to affect ribosome assembly. Although, in vitro reconstitution of bacterial ribosomal subunits can be achieved using purified ribosomal RNA (rRNA) and r-protein components, the process occurs at nonphysiological conditions. It is suggested that a specific order of processing and assembly events and regulatory factors is required to achieve fully functional particles. This article focuses on the protein factors that are involved in the process of bacterial ribosome biogenesis.

Key concepts:

  • This article focuses on the protein factors that are involved in the process of bacterial ribosome biogenesis.

Keywords:

  • assembly;
  • biogenesis;
  • ribosome;
  • RNA;
  • translation;
  • bacteria