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Fluorescence Spectroscopy In Vivo

Biomedical Spectroscopy

  1. Karthik Vishwanath,
  2. Nimmi Ramanujam

Published Online: 15 DEC 2011

DOI: 10.1002/9780470027318.a0102.pub2

Encyclopedia of Analytical Chemistry

Encyclopedia of Analytical Chemistry

How to Cite

Vishwanath, K. and Ramanujam, N. 2011. Fluorescence Spectroscopy In Vivo. Encyclopedia of Analytical Chemistry. .

Author Information

  1. Duke University, Durham, NC, USA

Publication History

  1. Published Online: 15 DEC 2011

Abstract

Diagnostic techniques based on optical spectroscopy have the potential to link biochemical and morphological properties of tissues to individual patient care. In particular, these techniques are fast, noninvasive, and quantitative. Furthermore, they can be used to elucidate key tissue features, such as cellular metabolic rate, vascularity, intravascular oxygenation, and alterations in tissue morphology. These tissue features can be interpreted to shed light on a variety of clinical problems, such as precancerous and cancerous growth and atherosclerosis. The goal of this article is to review development and application of optical spectroscopy in the ultraviolet (UV) and visible (VIS) spectral regions, as a diagnostic tool in clinical applications. Particular emphasis is placed on steady-state UV/VIS fluorescence spectroscopy for the detection of precancers and cancers, in vivo.