Standard Article

Magnetic Resonance in Medicine, High Resolution Ex Vivo

Biomedical Spectroscopy

  1. Ian C. Smith,
  2. Dorothea E. Blandford

Published Online: 15 SEP 2006

DOI: 10.1002/9780470027318.a0111

Encyclopedia of Analytical Chemistry

Encyclopedia of Analytical Chemistry

How to Cite

Smith, I. C. and Blandford, D. E. 2006. Magnetic Resonance in Medicine, High Resolution Ex Vivo. Encyclopedia of Analytical Chemistry. .

Author Information

  1. Institute for Biodiagnostics, Winnipeg, Canada

Publication History

  1. Published Online: 15 SEP 2006


Nuclear magnetic resonance (NMR) spectroscopy, or magnetic resonance spectroscopy (MRS), is a powerful physical technique to determine the quantity and structure of chemical compounds in a specimen. It involves the interaction of radiofrequency (RF) electromagnetic radiation with a collection of nuclei immersed in a strong magnetic field. It is unique in its ability to provide nondestructive chemical analyses in vivo and in vitro. Moreover, no or very little pretreatment of the specimen is required prior to making a measurement. Spectra take only a few minutes to acquire, and it is not necessary to preselect the metabolites of interest in order to detect and quantify them. Its limitations are a lack of availability of instruments and large databases of spectral changes correlated with pathological conditions, and a detection sensitivity of approximately 1 µM.