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Two-Dimensional Vibrational Correlation Spectroscopy in Biomedical Sciences

Biomedical Spectroscopy

  1. Yukihiro Ozaki1,
  2. Isao Noda2

Published Online: 15 SEP 2006

DOI: 10.1002/9780470027318.a0116

Encyclopedia of Analytical Chemistry

Encyclopedia of Analytical Chemistry

How to Cite

Ozaki, Y. and Noda, I. 2006. Two-Dimensional Vibrational Correlation Spectroscopy in Biomedical Sciences. Encyclopedia of Analytical Chemistry. .

Author Information

  1. 1

    Kwansei-Gakuin University, Nishinomiya, Japan

  2. 2

    The Procter and Gamble Company, Cincinnati, USA

Publication History

  1. Published Online: 15 SEP 2006


Two-dimensional (2-D) vibrational correlation spectroscopy offers a new opportunity for analyzing vibrational spectra. In the 2-D approach, spectral peaks are spread over the second dimension, thereby simplifying the visualization of complex spectra consisting of many overlapped bands, and enhancing spectral resolution. Correlation peaks appearing in the synchronous and asynchronous maps represent in-phase and out-of-phase variation tendencies of corresponding band intensities, respectively. 2-D correlation spectroscopy is very useful in unraveling infrared (IR), near-infrared (NIR), and Raman spectra of biological molecules and biomedical materials. For example, it has a powerful deconvolution ability for highly overlapped amide I, amide II, and amide III bands of proteins, enabling these bands to be assigned to various secondary structures. 2-D NIR correlation spectroscopy provides new insights into the hydration of proteins, and can also be used to investigate the order of perturbation-dependent structural changes.