Two-Dimensional Vibrational Correlation Spectroscopy in Biomedical Sciences
Published Online: 15 SEP 2006
Copyright © 2000 John Wiley & Sons, Ltd. All rights reserved.
Encyclopedia of Analytical Chemistry
How to Cite
Ozaki, Y. and Noda, I. 2006. Two-Dimensional Vibrational Correlation Spectroscopy in Biomedical Sciences. Encyclopedia of Analytical Chemistry. .
- Published Online: 15 SEP 2006
Two-dimensional (2-D) vibrational correlation spectroscopy offers a new opportunity for analyzing vibrational spectra. In the 2-D approach, spectral peaks are spread over the second dimension, thereby simplifying the visualization of complex spectra consisting of many overlapped bands, and enhancing spectral resolution. Correlation peaks appearing in the synchronous and asynchronous maps represent in-phase and out-of-phase variation tendencies of corresponding band intensities, respectively. 2-D correlation spectroscopy is very useful in unraveling infrared (IR), near-infrared (NIR), and Raman spectra of biological molecules and biomedical materials. For example, it has a powerful deconvolution ability for highly overlapped amide I, amide II, and amide III bands of proteins, enabling these bands to be assigned to various secondary structures. 2-D NIR correlation spectroscopy provides new insights into the hydration of proteins, and can also be used to investigate the order of perturbation-dependent structural changes.