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Proteoglycan and Acidic Polysaccharide Analysis

Carbohydrate Analysis

  1. Robert M. Lauder

Published Online: 15 SEP 2006

DOI: 10.1002/9780470027318.a0310

Encyclopedia of Analytical Chemistry

Encyclopedia of Analytical Chemistry

How to Cite

Lauder, R. M. 2006. Proteoglycan and Acidic Polysaccharide Analysis. Encyclopedia of Analytical Chemistry. .

Author Information

  1. Lancaster University, Lancaster, UK

Publication History

  1. Published Online: 15 SEP 2006

Abstract

The isolation and analysis of proteoglycans (PGs) and the acidic polysaccharide glycosaminoglycan (GAG) chains attached to them is an area of increasing importance. PGs are a structurally diverse group of complex macromolecules comprising a core protein, varying in size from 11 to 400 kDa, to which are attached one or more GAG chains of the same or a different type. These chains carry a negative charge derived mainly from sulfate groups and are composed of a repeating disaccharide, a hexosamine and either a hexuronic acid or, in keratan sulfate (KS), galactose.