Standard Article

Proteoglycan and Acidic Polysaccharide Analysis

Carbohydrate Analysis

  1. Robert M. Lauder

Published Online: 15 SEP 2006

DOI: 10.1002/9780470027318.a0310

Encyclopedia of Analytical Chemistry

Encyclopedia of Analytical Chemistry

How to Cite

Lauder, R. M. 2006. Proteoglycan and Acidic Polysaccharide Analysis. Encyclopedia of Analytical Chemistry. .

Author Information

  1. Lancaster University, Lancaster, UK

Publication History

  1. Published Online: 15 SEP 2006

This is not the most recent version of the article. View current version (10 SEP 2015)


The isolation and analysis of proteoglycans (PGs) and the acidic polysaccharide glycosaminoglycan (GAG) chains attached to them is an area of increasing importance. PGs are a structurally diverse group of complex macromolecules comprising a core protein, varying in size from 11 to 400 kDa, to which are attached one or more GAG chains of the same or a different type. These chains carry a negative charge derived mainly from sulfate groups and are composed of a repeating disaccharide, a hexosamine and either a hexuronic acid or, in keratan sulfate (KS), galactose.