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Phospholipids of Plasma Lipoproteins, Red Blood Cells and Atheroma, Analysis of

Clinical Chemistry

  1. Amir Ravandi,
  2. Arnis Kuksis

Published Online: 15 SEP 2006

DOI: 10.1002/9780470027318.a0530

Encyclopedia of Analytical Chemistry

Encyclopedia of Analytical Chemistry

How to Cite

Ravandi, A. and Kuksis, A. 2006. Phospholipids of Plasma Lipoproteins, Red Blood Cells and Atheroma, Analysis of. Encyclopedia of Analytical Chemistry. .

Author Information

  1. University of Toronto, Toronto, Canada

Publication History

  1. Published Online: 15 SEP 2006

Abstract

The large number of lipid classes and great complexity of molecular species present in blood plasma, red cells, platelets and atheromatous lesions requires a combination of analytical techniques for comprehensive analyses, including chemical and enzymatic derivatization of samples. In many instances only partial analyses are required, which can be accomplished by specific analytical techniques. This chapter describes the full spectrum of the methodology ranging from the most basic thin-layer chromatography (TLC) to the detailed mass spectrometric assays. Traditional sample extraction by liquid–liquid partition is time-consuming and involves large volumes of solvents. Liquid–solid extraction using adsorbent cartridges is more economical. At the present time total lipid extracts can be effectively assayed for lipid class content and molecular species composition by flow injection tandem mass spectrometry (MS/MS), while a more detailed analysis of complex lipid mixtures is provided by a combination of liquid chromatography with on-line electrospray mass spectrometry (LC/ESMS). The new techniques permit completion of the analyses in a few hours, where previously several days or weeks may have been required. The soft ionization mass spectrometric techniques have permitted the recognition and detailed analysis of such minor components of blood as the lipid oxidation and glycation products often observed in disease.