Gas Chromatography and Mass Spectrometry in Clinical Chemistry
Published Online: 15 SEP 2006
Copyright © 2000 John Wiley & Sons, Ltd. All rights reserved.
Encyclopedia of Analytical Chemistry
How to Cite
Bertholf, R. L. 2006. Gas Chromatography and Mass Spectrometry in Clinical Chemistry. Encyclopedia of Analytical Chemistry.
- Published Online: 15 SEP 2006
Automated spectrophotometric, electrochemical, and immunochemical analyses have become the mainstays of clinical chemistry and toxicology laboratory services, but their scope is limited. A wide array of clinically relevant analytes demand more sophisticated analytical techniques to provide sensitive and specific assays for diagnostic purposes. Gas chromatography (GC) and mass spectrometry (MS) are becoming cost-effective alternatives for many of these challenging assays. GC is a robust technique that offers the ability to resolve volatile components of a complex mixture. MS provides structural information that can unambiguously identify a compound. In combination, these two techniques provide qualitative and quantitative answers to many difficult analytical problems. GC and MS applications have been developed for a variety of clinical analytes, and the use of these methods has created new and exciting frontiers for clinical laboratory medicine. Several clinical applications of these analytical techniques will be discussed in this chapter.