Standard Article

Nucleic Acid Analysis in Clinical Chemistry

Clinical Chemistry

  1. Michael Thompson,
  2. Anil K. Deisingh

Published Online: 15 SEP 2006

DOI: 10.1002/9780470027318.a0536

Encyclopedia of Analytical Chemistry

Encyclopedia of Analytical Chemistry

How to Cite

Thompson, M. and Deisingh, A. K. 2006. Nucleic Acid Analysis in Clinical Chemistry. Encyclopedia of Analytical Chemistry. .

Author Information

  1. University of Toronto, Toronto, Canada

Publication History

  1. Published Online: 15 SEP 2006


This article examines the role of nucleic acid analysis in clinical chemistry. It is a broad-based article and begins with the background knowledge which will be needed to appreciate the succeeding sections. In the Introduction, structure and function of nucleic acids, hybridization, probe labeling, hybridization protection, polymerase chain reaction (PCR) and sequencing are discussed. Major sections are devoted to microbiological analysis (with particular emphasis on bacteriology), genetic diseases and screening [discussions are provided for cystic fibrosis (CF), heriditary fructose intolerance (HFI) and hemochromatosis along with an introduction to the Human Genome Project and single nucleotide polymorphisms (SNP)], other areas of human medicine (oncology, colorectal cancer and Alzheimer's disease (AD) are considered) and the biosensor approach. A discussion of interesting current and future developments, not only in nucleic acid analysis, but also in gene therapy, concludes the article.