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Ultraviolet/Visible Light Absorption Spectrophotometry in Clinical Chemistry

Clinical Chemistry

  1. Stephen L. Upstone

Published Online: 15 MAR 2013

DOI: 10.1002/9780470027318.a0547.pub2

Encyclopedia of Analytical Chemistry

Encyclopedia of Analytical Chemistry

How to Cite

Upstone, S. L. 2013. Ultraviolet/Visible Light Absorption Spectrophotometry in Clinical Chemistry . Encyclopedia of Analytical Chemistry. .

Author Information

  1. PerkinElmer Inc., Seer Green, UK

Publication History

  1. Published Online: 15 MAR 2013

Abstract

Ultraviolet/visible (UV/VIS) absorption spectroscopy has been used for clinical analysis for many years. The technique has appeal, as it is almost universal in its application. Although much of the routine work is performed using high-throughput dedicated clinical analysis systems, absorption spectroscopy still has a place in most clinical laboratories. This article discusses the range of application types for which absorption spectroscopy can be used and provides some examples of common analyses. The article also discusses the merits of various instrument types and deals with some more advanced spectroscopic techniques, such as derivative spectroscopy, to enhance the data measured by the spectrophotometer. Brief reference is also made to the use of reflectance in clinical analysis.