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Ultraviolet/Visible Light Absorption Spectrophotometry in Clinical Chemistry

Clinical Chemistry

  1. Stephen L. Upstone

Published Online: 15 SEP 2006

DOI: 10.1002/9780470027318.a0547

Encyclopedia of Analytical Chemistry

Encyclopedia of Analytical Chemistry

How to Cite

Upstone, S. L. 2006. Ultraviolet/Visible Light Absorption Spectrophotometry in Clinical Chemistry. Encyclopedia of Analytical Chemistry. .

Author Information

  1. PerkinElmer Ltd., Beaconsfield, UK

Publication History

  1. Published Online: 15 SEP 2006

This is not the most recent version of the article. View current version (15 MAR 2013)


Ultraviolet/visible (UV/VIS) absorption spectroscopy has been used in the clinical laboratory for many years. The technique has appeal, as it is almost universal in its application. Although much of the routine work is performed using high-throughput dedicated clinical analysis systems, absorption spectroscopy still has a place in most clinical laboratories.

This article discusses the range of application types for which absorption spectroscopy can be used and some examples of common analyses are given. The article also discusses the merits of various instrument types and discusses some more advanced spectroscopic techniques, such as derivative spectroscopy, to enhance the data measured by the spectrophotometer. Brief reference is also made to the use of reflectance in clinical analysis.