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Aptamers

Nucleic Acids Structure and Mapping

  1. Subash C. B. Gopinath

Published Online: 15 MAR 2011

DOI: 10.1002/9780470027318.a1402.pub2

Encyclopedia of Analytical Chemistry

Encyclopedia of Analytical Chemistry

How to Cite

Gopinath, S. C. B. 2011. Aptamers. Encyclopedia of Analytical Chemistry. .

Author Information

  1. National Institute of Advanced Industrial Science and Technology, Tsukuba, Japan

  1. Update based on original article by William James, Encyclopedia of Analytical Chemistry, © 2000, John Wiley & Sons Ltd.

Publication History

  1. Published Online: 15 MAR 2011

Abstract

Aptamers are artificial nucleic acid ligands that can be generated against a wide range of targets including amino acids, drugs, proteins, and other molecules. They are isolated from complex libraries of synthetic nucleic acid by an iterative process of adsorption, recovery, and reamplification. They have potential applications in analytical sensing devices, target validation, and as therapeutic agents. Aptamers are now widely accepted as substitutes for antibodies, especially in the diagnostic front. Aptamers are approved as drugs for therapeutics, and several aptamers are under clinical trials with the ultimate aim for commercialization.

Keywords:

  • aptamer;
  • SELEX;
  • artificial-ligands;
  • in vitro selection;
  • combinatorial library