Radiation Hybrid Mapping
Nucleic Acids Structure and Mapping
Published Online: 15 SEP 2006
Copyright © 2000 John Wiley & Sons, Ltd. All rights reserved.
Encyclopedia of Analytical Chemistry
How to Cite
Newell, W. R. 2006. Radiation Hybrid Mapping. Encyclopedia of Analytical Chemistry. .
- Published Online: 15 SEP 2006
Radiation hybrid (RH) mapping is a convenient and widely used method for rapidly locating new DNA sequences on genomes in relation to other DNA markers. The aim of the technique is to determine the correct order and relative distances between a set of markers, or to locate a new marker to its most likely location on a genome. We review the history of the technique, its current status, and the main analytical techniques used to generate maps from raw data. The physical processes and assumptions underlying the technique are examined. These processes are used to derive models from which the required information, location of markers with respect to each other on a linear map of DNA, is obtained by a variety of computational means. The RH technique has proved indispensable in mapping the human genome, since, unlike genetic mapping, the conditions under which panels are made is completely under experimental control, so mapping at any degree of resolution is possible. The other major advantage of RH mapping over genetic mapping is that markers need not be polymorphic; it is presence or absence of a marker that is detected, not type. It has therefore been used to map the large numbers of expressed sequence tags that have been generated as part of the human genome project (HGP). This has given rise to the first human gene map and subsequent updates, so that currently 40 000 genes have been assigned locations on the human genome.