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Capillary Electrophoresis in Peptide and Protein Analysis, Detection Modes for

Peptides and Proteins

  1. Paul L. Weber

Published Online: 15 SEP 2006

DOI: 10.1002/9780470027318.a1603

Encyclopedia of Analytical Chemistry

Encyclopedia of Analytical Chemistry

How to Cite

Weber, P. L. 2006. Capillary Electrophoresis in Peptide and Protein Analysis, Detection Modes for. Encyclopedia of Analytical Chemistry. .

Author Information

  1. Briar Cliff College, Sioux City, USA

Publication History

  1. Published Online: 15 SEP 2006

Abstract

Capillary electrophoresis (CE) is a highly efficient separation technique which achieves high resolution with low sample consumption. Crucial to any separation technique are detection modes capable of giving useful data about separated analytes, including both quantitative and qualitative information. A variety of general detection modes for CE have been developed, most based on similar systems used in high-performance liquid chromatography (HPLC), but only some are useful in the analysis of peptides and proteins. Modes which are selective for certain spectral or chemical properties of these analytes are often employed. The absorption of ultraviolet/visible (UV/VIS) light as well as fluorescence (Fl) fit into the former category while electrochemical (EC) detection falls under the latter. The development of mass spectrometry (MS) as a detection mode for peptide and protein analysis may be the ideal choice since a great deal of qualitative information can be obtained and used for identification and structural characterization. By definition, CE involves separation in a “micro” environment, a capillary, and this places certain demands on the performance of the detection system and perhaps the greatest challenge is that of sensitivity.