Capillary Electrophoresis of Proteins and Glycoproteins
Peptides and Proteins
Published Online: 15 SEP 2006
Copyright © 2000 John Wiley & Sons, Ltd. All rights reserved.
Encyclopedia of Analytical Chemistry
How to Cite
Trout, A., Muza, M. M. and Landers, J. P. 2006. Capillary Electrophoresis of Proteins and Glycoproteins. Encyclopedia of Analytical Chemistry. .
- Published Online: 15 SEP 2006
As a result of their complex nature, the separation of proteins and glycoproteins by capillary electrophoresis (CE) is a challenge. Fortunately, a number of approaches allow for effective analysis of these biomolecules. Of particular concern is the proclivity of proteins and their glycoconjugates to adsorb to the capillary wall. Various covalent and dynamic capillary coatings are described which minimize this effect. We also address general concerns including electrode polarity, capillary conditioning and method development that are important for achieving optimal separation. Finally, each separation mode, including capillary zone electrophoresis (CZE), capillary gel electrophoresis (CGE), and capillary isoelectric focusing (CIEF), is discussed with particular attention to its niche in the realm of protein and glycoprotein analysis.