Standard Article

Fluorescence Spectroscopy in Peptide and Protein Analysis

Peptides and Proteins

  1. Alexey S. Ladokhin

Published Online: 15 SEP 2006

DOI: 10.1002/9780470027318.a1611

Encyclopedia of Analytical Chemistry

Encyclopedia of Analytical Chemistry

How to Cite

Ladokhin, A. S. 2006. Fluorescence Spectroscopy in Peptide and Protein Analysis. Encyclopedia of Analytical Chemistry. .

Author Information

  1. University of California, Irvine, USA

Publication History

  1. Published Online: 15 SEP 2006

Abstract

Fluorescence spectroscopy and its multiple applications to the life sciences have undergone rapid development. This is due to numerous technical advances in both instrumentation and methods of data analysis as well as to a vast proliferation of basic techniques. Applications of fluorescence spectroscopy to protein and peptide analysis are governed by three principal factors: the dynamic nature of the signal, its localized nature, and its redundancy. Although these features can complicate interpretation of the experimental result, they also can be exploited to obtain unique structural and dynamic information. The availability and simplicity of basic data acquisition and analysis are important practical features behind the popularity of fluorescence as compared to other spectroscopic techniques. Yet this simplicity does not appear to compromise its advantages. This article is intended first to provide an overview of the fluorescence phenomenon in proteins, and second to illustrate applications of fluorescence spectroscopy in advanced (but not necessarily high-tech) studies. Three areas of protein studies, namely protein–ligand interactions, protein folding and studies of membrane proteins, have been chosen to demonstrate key advantages of fluorescence spectroscopy: it is sensitive, versatile, and it lends itself readily to fast data acquisition.