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Surface Plasmon Resonance Spectroscopy in Peptide and Protein Analysis

Peptides and Proteins

  1. Zdzislaw Salamon,
  2. Gordon Tollin

Published Online: 15 SEP 2006

DOI: 10.1002/9780470027318.a1633

Encyclopedia of Analytical Chemistry

Encyclopedia of Analytical Chemistry

How to Cite

Salamon, Z. and Tollin, G. 2006. Surface Plasmon Resonance Spectroscopy in Peptide and Protein Analysis. Encyclopedia of Analytical Chemistry. .

Author Information

  1. University of Arizona, Tucson, USA

Publication History

  1. Published Online: 15 SEP 2006

Abstract

This chapter presents a detailed overview of the theoretical basis of surface plasmon resonance (SPR) spectroscopy, emphasizing both principles and practical considerations such as thin-film designs for SPR devices which can be employed in characterization of biological materials, including peptides and proteins. It is demonstrated that the analysis of the resonance spectrum yields structural parameters of anisotropic films of interacting biomolecules, including distribution of mass, long-range molecular order, and molecular conformational changes. Binding parameters, including binding affinities and rate constants, can also be determined. A summary is presented of different techniques for biomolecule immobilization on SPR-generating surfaces applicable in peptide and protein analysis.