Surface Plasmon Resonance Spectroscopy in Peptide and Protein Analysis
Peptides and Proteins
Published Online: 15 SEP 2006
Copyright © 2000 John Wiley & Sons, Ltd. All rights reserved.
Encyclopedia of Analytical Chemistry
How to Cite
Salamon, Z. and Tollin, G. 2006. Surface Plasmon Resonance Spectroscopy in Peptide and Protein Analysis. Encyclopedia of Analytical Chemistry. .
- Published Online: 15 SEP 2006
This chapter presents a detailed overview of the theoretical basis of surface plasmon resonance (SPR) spectroscopy, emphasizing both principles and practical considerations such as thin-film designs for SPR devices which can be employed in characterization of biological materials, including peptides and proteins. It is demonstrated that the analysis of the resonance spectrum yields structural parameters of anisotropic films of interacting biomolecules, including distribution of mass, long-range molecular order, and molecular conformational changes. Binding parameters, including binding affinities and rate constants, can also be determined. A summary is presented of different techniques for biomolecule immobilization on SPR-generating surfaces applicable in peptide and protein analysis.