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Planar Chromatography in Pharmaceutical Analysis

Pharmaceuticals and Drugs

  1. A.P. Argekar

Published Online: 15 SEP 2006

DOI: 10.1002/9780470027318.a1916

Encyclopedia of Analytical Chemistry

Encyclopedia of Analytical Chemistry

How to Cite

Argekar, A. 2006. Planar Chromatography in Pharmaceutical Analysis. Encyclopedia of Analytical Chemistry. .

Author Information

  1. The Institute of Science, Mumbai, India

Publication History

  1. Published Online: 15 SEP 2006


Planar chromatography is a liquid chromatography in which the stationary phase is arranged in the form of a planar or flat bed and the mobile phase moves by capillary action. Thin-layer chromatography (TLC) is the most widely used planar chromatography. Modern TLC is an instrumental version of conventional TLC known as high-performance thin-layer chromatography (HPTLC). Rotation planar chromatography (RPL), high-speed thin-layer chromatography (HSTLC), and over-pressured liquid chromatography (OPLC) are some other forms of planar chromatographic methods.

The application of HPTLC techniques for pharmaceutical analysis, such as the determination of active pharmaceutical ingredients (API) and the content uniformity test (CUT) of dosages, purity and stability tests of drugs and drug products, process monitoring of formulations, and herbal drug analysis, is described in this article. The method development of the high-performance liquid chromatography (HPLC) technique, various pharmaceutical regulations and methods, validation and quality assurance (QA) of HPTLC experimental data and documentation are also described. The comparison of HPTLC with other chromatographic techniques is also discussed.