Planar Chromatography in Pharmaceutical Analysis
Pharmaceuticals and Drugs
Published Online: 15 SEP 2006
Copyright © 2000 John Wiley & Sons, Ltd. All rights reserved.
Encyclopedia of Analytical Chemistry
How to Cite
Argekar, A. 2006. Planar Chromatography in Pharmaceutical Analysis. Encyclopedia of Analytical Chemistry. .
- Published Online: 15 SEP 2006
Planar chromatography is a liquid chromatography in which the stationary phase is arranged in the form of a planar or flat bed and the mobile phase moves by capillary action. Thin-layer chromatography (TLC) is the most widely used planar chromatography. Modern TLC is an instrumental version of conventional TLC known as high-performance thin-layer chromatography (HPTLC). Rotation planar chromatography (RPL), high-speed thin-layer chromatography (HSTLC), and over-pressured liquid chromatography (OPLC) are some other forms of planar chromatographic methods.
The application of HPTLC techniques for pharmaceutical analysis, such as the determination of active pharmaceutical ingredients (API) and the content uniformity test (CUT) of dosages, purity and stability tests of drugs and drug products, process monitoring of formulations, and herbal drug analysis, is described in this article. The method development of the high-performance liquid chromatography (HPLC) technique, various pharmaceutical regulations and methods, validation and quality assurance (QA) of HPTLC experimental data and documentation are also described. The comparison of HPTLC with other chromatographic techniques is also discussed.