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Proteins and Peptides Purification in Pharmaceuticals Analysis

Pharmaceuticals and Drugs

  1. Marvin E. Thrash Jr,
  2. Neville G. Pinto

Published Online: 15 SEP 2006

DOI: 10.1002/9780470027318.a1917

Encyclopedia of Analytical Chemistry

Encyclopedia of Analytical Chemistry

How to Cite

Thrash, M. E. and Pinto, N. G. 2006. Proteins and Peptides Purification in Pharmaceuticals Analysis. Encyclopedia of Analytical Chemistry. .

Author Information

  1. University of Cincinnati, Department of Chemical Engineering, USA

Publication History

  1. Published Online: 15 SEP 2006


The biopharmaceutical market has exploded in the last 10 years, with annual sales easily exceeding a billion dollars per year. Analysis and purification are essential components in the production of therapeutic proteins and peptides, and chromatography is at the core of all pharmaceutical separations. The more popular chromatographic techniques used in the purification of proteins and peptides are reversed-phase chromatography (RPC), hydrophobic interaction chromatography (HIC), ion-exchange chromatography (IEC), affinity chromatography (AC) and size-exclusion chromatography (SEC). In order to meet the challenges of the future it is essential to understand the fundamentals of each technique. In this article an overview of the more popular chromatographic techniques employed to purify biomolecules is presented. Relevant examples are also given and a discussion of detection and scale-up is provided.