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Solid-Phase Extraction and Clean-Up Procedures in Pharmaceutical Analysis

Pharmaceuticals and Drugs

  1. P. Campíns-Falcó,
  2. A. Sevillano-Cabeza,
  3. R. Herráez-Hernández,
  4. C. Molins-Legua,
  5. Y. Moliner-Martínez,
  6. J. Verdú-Andrés

Published Online: 17 DEC 2012

DOI: 10.1002/9780470027318.a1920.pub2

Encyclopedia of Analytical Chemistry

Encyclopedia of Analytical Chemistry

How to Cite

Campíns-Falcó, P., Sevillano-Cabeza, A., Herráez-Hernández, R., Molins-Legua, C., Moliner-Martínez, Y. and Verdú-Andrés, J. 2012. Solid-Phase Extraction and Clean-Up Procedures in Pharmaceutical Analysis . Encyclopedia of Analytical Chemistry. .

Author Information

  1. Universitat de Valencia, Valencia, Spain

Publication History

  1. Published Online: 17 DEC 2012
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Figure 1. Timeline with the main contributions to the development of SPE: sorbents (left) and formats (right).

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Figure 2. Typical scheme of magnetic SPE.

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Figure 3. Typical scheme of a column-switching system used for on-line SPE.

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Figure 4. Set-up for on-line SPC/GC (a), and the chromatograms obtained from 1 mL of plasma spiked with 100 ng mL−1 of trazodone (T) (b) and blank plasma (c) V1, V2, and V3 are switching valves; SVE, solvent vapor exit; FID, flame ionization detector (reproduced by permission of Vieweg Publishing from Ref. 32.)

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Figure 5. Typical configurations of IT-SPME: extraction with packed capillary columns (a), extraction by draw/eject cycles (b), and in-valve IT-SPME (c).

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Figure 6. Typical chromatograms obtained at 230 nm from a urine sample by (a) acidic LLE, (b) basic LLE, (c) off-line SPE into a C18 column cartridge, and (d) on-line SPE with column switching using a precolumn packed with a C18 packing.

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Figure 7. Effect of the number of injections on the back-pressure of the precolumn (+), the analytical column (×), and on the two columns linked (•). The dashed line indicates cleaning both columns with acetonitrile (approximately 15 mL).