Standard Article

Chemiluminescence, Electrogenerated

Electroanalytical Methods

  1. Allen J. Bard1,
  2. Jeff D. Debad2,
  3. Jonathan K. Leland2,
  4. George B. Sigal2,
  5. James L. Wilbur2,
  6. Jacob N. Wohlstadter2

Published Online: 15 SEP 2006

DOI: 10.1002/9780470027318.a5302

Encyclopedia of Analytical Chemistry

Encyclopedia of Analytical Chemistry

How to Cite

Bard, A. J., Debad, J. D., Leland, J. K., Sigal, G. B., Wilbur, J. L. and Wohlstadter, J. N. 2006. Chemiluminescence, Electrogenerated. Encyclopedia of Analytical Chemistry. .

Author Information

  1. 1

    University of Texas at Austin, Austin, USA

  2. 2

    IGEN International, Inc., Gaithersburg, USA

Publication History

  1. Published Online: 15 SEP 2006

This is not the most recent version of the article. View current version (10 JAN 2014)


Electrogenerated chemiluminescence (ECL) is the process in which electrogenerated species undergo electron transfer reactions to form excited states that emit light. Many molecules have the potential to produce ECL, however Ru(bpy)32+ (bpy = 2,2′-bipyridine) is the most common emitter used for analytical applications. Application of a voltage to an electrode in the presence of an emitter induces light production and allows for the detection of the emitter at very low concentrations. Advantages over other analytical methods include low backgrounds, precise spatial and temporal control over the emission, and the possibility of signal amplification. Commercial systems exist that use ECL to detect numerous clinically relevant analytes with high sensitivity using a variety of assay formats.