Published Online: 15 SEP 2006
Copyright © 2000 John Wiley & Sons, Ltd. All rights reserved.
Encyclopedia of Analytical Chemistry
How to Cite
Bard, A. J., Debad, J. D., Leland, J. K., Sigal, G. B., Wilbur, J. L. and Wohlstadter, J. N. 2006. Chemiluminescence, Electrogenerated. Encyclopedia of Analytical Chemistry. .
- Published Online: 15 SEP 2006
This is not the most recent version of the article. View current version (10 JAN 2014)
Electrogenerated chemiluminescence (ECL) is the process in which electrogenerated species undergo electron transfer reactions to form excited states that emit light. Many molecules have the potential to produce ECL, however Ru(bpy)32+ (bpy = 2,2′-bipyridine) is the most common emitter used for analytical applications. Application of a voltage to an electrode in the presence of an emitter induces light production and allows for the detection of the emitter at very low concentrations. Advantages over other analytical methods include low backgrounds, precise spatial and temporal control over the emission, and the possibility of signal amplification. Commercial systems exist that use ECL to detect numerous clinically relevant analytes with high sensitivity using a variety of assay formats.