Fluorescence Imaging Microscopy
Electronic Absorption and Luminescence
Published Online: 15 DEC 2011
Copyright © 2000 John Wiley & Sons, Ltd. All rights reserved.
Encyclopedia of Analytical Chemistry
How to Cite
Lemasters, J. J. 2011. Fluorescence Imaging Microscopy . Encyclopedia of Analytical Chemistry. .
- Published Online: 15 DEC 2011
Fluorescence imaging microscopy is a powerful tool to localize specific molecular components with lateral resolution of 0.2 µm. New techniques of confocal and multiphoton microscopy also permit submicrometer axial resolution even in thick specimens, and novel superresolution techniques that improve resolution to 0.1 µm and less are emerging. The number of specific parameter-indicating fluorophores useful for nondestructive live cell imaging continues to increase, including markers of cell shape and volume, organelles, membrane potentials, ions, and other chemical constitutents. Moreover, virtually, any cellular protein can be tagged with a fluorescent protein in the living state. These developments make fluorescence microscopy an indispensable tool for the molecular biologist, cell biologist, physiologist, and others both in and not in the life sciences.