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Fluorescence Imaging Microscopy

Electronic Absorption and Luminescence

  1. John J. Lemasters

Published Online: 15 DEC 2011

DOI: 10.1002/9780470027318.a5406.pub2

Encyclopedia of Analytical Chemistry

Encyclopedia of Analytical Chemistry

How to Cite

Lemasters, J. J. 2011. Fluorescence Imaging Microscopy . Encyclopedia of Analytical Chemistry. .

Author Information

  1. Medical University of South Carolina, Charleston, SC, USA

Publication History

  1. Published Online: 15 DEC 2011

Abstract

Fluorescence imaging microscopy is a powerful tool to localize specific molecular components with lateral resolution of 0.2 µm. New techniques of confocal and multiphoton microscopy also permit submicrometer axial resolution even in thick specimens, and novel superresolution techniques that improve resolution to 0.1 µm and less are emerging. The number of specific parameter-indicating fluorophores useful for nondestructive live cell imaging continues to increase, including markers of cell shape and volume, organelles, membrane potentials, ions, and other chemical constitutents. Moreover, virtually, any cellular protein can be tagged with a fluorescent protein in the living state. These developments make fluorescence microscopy an indispensable tool for the molecular biologist, cell biologist, physiologist, and others both in and not in the life sciences.