Fluorescence Lifetime Measurements, Applications of
Electronic Absorption and Luminescence
Published Online: 15 SEP 2006
Copyright © 2000 John Wiley & Sons, Ltd. All rights reserved.
Encyclopedia of Analytical Chemistry
How to Cite
Bright, F. V., Pandey, S. and Baker, G. A. 2006. Fluorescence Lifetime Measurements, Applications of. Encyclopedia of Analytical Chemistry. .
- Published Online: 15 SEP 2006
Over the years, the emissive characteristics (spectral, temporal, and polarization) of fluorophores have been widely used to probe the local environments of fluorescent centers. Fluorescence lifetime and rotational reorientation time measurements, in particular, offer a means to elucidate subtle features when examining, for example, complex systems involving environmental heterogeneity, energy transfer, quenching pathways, or excited state reactions. Further, because fluorescence occurs on the nanosecond (10−9 s) timescale, competing or perturbing kinetic processes like collisional quenching, solvent relaxation, energy transfer, and rotational reorientation can affect the fluorescence. Thus, a carefully chosen fluorophore can serve as an internal nanosecond or faster stopwatch of environmental fluctuations.
This article is divided into a brief introduction, a short discussion on instrumentation, and several sections on various applications of excited state fluorescence measurements.