Standard Article

Fluorescence in Organized Assemblies

Electronic Absorption and Luminescence

  1. Najma Memon,
  2. Aamna Balouch,
  3. Willie L. Hinze

Published Online: 29 SEP 2008

DOI: 10.1002/9780470027318.a5409.pub2

Encyclopedia of Analytical Chemistry

Encyclopedia of Analytical Chemistry

How to Cite

Memon, N., Balouch, A. and Hinze, W. L. 2008. Fluorescence in Organized Assemblies. Encyclopedia of Analytical Chemistry. .

Author Information

  1. Wake Forest University, Winston-Salem, NC, USA

Publication History

  1. Published Online: 29 SEP 2008

Abstract

This article provides an overview of the general properties of organized assembly (ordered media) systems, such as aqueous surfactant and bile salt micelles, lipid (liposomes) and surfactant vesicles, and cyclodextrins (CDs), and summarizes their utilization to enhance the performance of analytical fluorescence measurements. In many instances, organic molecules and metal complex species when included within a CD cavity or solubilized and bound to surfactant aggregates exhibit enhanced fluorescence, improving detectability of such analytes. The altered microenvironment within the organized medium can impede the interfering action of other species (inorganic or organic) present in the sample matrix, which often improves the selectivity of the analytical method. These benefits of improved sensitivity and selectivity arise from the compartmentalization, isolation, and shielding of the excited singlet state of the guest analyte from quenching and nonradiative decay processes, as well as preventing side reactions that otherwise can occur in bulk solution (or sample matrix). Organic solvents or time-consuming extraction steps can also be avoided, owing to the increased solubility of nonpolar organic or inorganic reagents and/or analyte molecules in water in the presence of the organized medium, allowing the use of an aqueous medium to perform the procedure. The possibility of conducting reactions and forming fluorescent organic or metal chelates in micellar (or other organized) media that are not observed in a bulk homogeneous solvent system serves to expand the scope of possible chemistries for design/development of new, unique, and improved fluorescent assays. Examples of fluorescent methods for determination of both organic and inorganic analytes are provided, which serve to illustrate the advantages and benefits accrued from the use of the micelles, vesicles, liposomes, or CDs in such procedures, along with experimental considerations and cautions in utilizing organized media.