Absorption and Luminescence Probes
Electronic Absorption and Luminescence
Published Online: 15 JUN 2010
Copyright © 2000 John Wiley & Sons, Ltd. All rights reserved.
Encyclopedia of Analytical Chemistry
How to Cite
Rusin, O., Strongin, R. M. and Lim, S. 2010. Absorption and Luminescence Probes. Encyclopedia of Analytical Chemistry. .
- Published Online: 15 JUN 2010
Absorption and luminescent probes are compounds with an extensive variety of molecular structures. Despite their diversity and variations in their properties, they possess many similar general features. In solution they can form specific complexes with targets, leading to corresponding measurable changes in photophysical properties. Depending on the particular research need, there is a large choice of available probes absorbing and emitting light in ultraviolet (UV), visible, or near infrared (NIR) spectral ranges. They have been commonly used, for example, for pH measurements, the detection of gases, cations or anions, in the screening of metabolites, in monitoring macromolecular transformations, as well as in numerous other related chemical and biochemical processes.