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Affinity Chromatography

Liquid Chromatography

  1. David S. Hage

Published Online: 15 JUN 2012

DOI: 10.1002/9780470027318.a5902.pub2

Encyclopedia of Analytical Chemistry

Encyclopedia of Analytical Chemistry

How to Cite

Hage, D. S. 2012. Affinity Chromatography. Encyclopedia of Analytical Chemistry. .

Author Information

  1. University of Nebraska, Chemistry Department, Lincoln, NE, USA

Publication History

  1. Published Online: 15 JUN 2012


Affinity chromatography is a type of liquid chromatography that makes use of biological interactions for the separation and specific analysis of sample components. This article discusses the basic principles behind the theory and use of this method. Various factors that are important in the development of an affinity chromatographic system are considered, such as the choice of affinity ligand, support material, immobilization method, and elution conditions. A survey of the different types of affinity chromatography is also presented; this includes bioaffinity chromatography, immunoaffinity chromatography, dye–ligand affinity chromatography, immobilized metal ion affinity chromatography, and analytical affinity chromatography. In addition, some areas of expected future growth are considered, such as the use of molecular imprints or aptamers as ligands in affinity columns and the coupling of affinity chromatography with other analytical techniques.