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Column Theory and Resolution in Liquid Chromatography

Liquid Chromatography

  1. Paul R. Haddad

Published Online: 15 DEC 2009

DOI: 10.1002/9780470027318.a5906.pub2

Encyclopedia of Analytical Chemistry

Encyclopedia of Analytical Chemistry

How to Cite

Haddad, P. R. 2009. Column Theory and Resolution in Liquid Chromatography. Encyclopedia of Analytical Chemistry. .

Author Information

  1. University of Tasmania, Hobart, Australia

Publication History

  1. Published Online: 15 DEC 2009

Abstract

Fundamental theory of liquid chromatography is covered, including the thermodynamic and kinetic processes underlying chromatographic retention. Key chromatographic terms are defined. Processes which lead to band-broadening phenomena and resultant loss of separation efficiency are then discussed. Next, factors which influence the resolution between analyte peaks are outlined in a discussion of the resolution equation. Finally, peak capacity is discussed in terms of its effect on the probability that a desired separation will be achieved.

Keywords:

  • chromatography;
  • gas chromatography;
  • liquid chromatography;
  • high-performance liquid chromatography;
  • high-pressure liquid chromatography