Column Theory and Resolution in Liquid Chromatography
Published Online: 15 DEC 2009
Copyright © 2000 John Wiley & Sons, Ltd. All rights reserved.
Encyclopedia of Analytical Chemistry
How to Cite
Haddad, P. R. 2009. Column Theory and Resolution in Liquid Chromatography. Encyclopedia of Analytical Chemistry. .
- Published Online: 15 DEC 2009
Fundamental theory of liquid chromatography is covered, including the thermodynamic and kinetic processes underlying chromatographic retention. Key chromatographic terms are defined. Processes which lead to band-broadening phenomena and resultant loss of separation efficiency are then discussed. Next, factors which influence the resolution between analyte peaks are outlined in a discussion of the resolution equation. Finally, peak capacity is discussed in terms of its effect on the probability that a desired separation will be achieved.
- gas chromatography;
- liquid chromatography;
- high-performance liquid chromatography;
- high-pressure liquid chromatography