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Reversed Phase Liquid Chromatography

Liquid Chromatography

  1. W. John Lough

Published Online: 15 SEP 2006

DOI: 10.1002/9780470027318.a5914

Encyclopedia of Analytical Chemistry

Encyclopedia of Analytical Chemistry

How to Cite

Lough, W. J. 2006. Reversed Phase Liquid Chromatography. Encyclopedia of Analytical Chemistry. .

Author Information

  1. Institute of Pharmacy & Chemistry, University of Sunderland, UK

Publication History

  1. Published Online: 15 SEP 2006


Reversed-phase liquid chromatography (RPLC) is an elution procedure used in liquid chromatography (LC) in which the mobile phase is significantly more polar than the stationary phase, e.g. a microporous silica-based material with chemically bonded alkyl chains. Typically, the mobile phase is a mixture of an aqueous component, often containing a buffer to control pH, and a water-miscible polar organic solvent. This most frequently used mode of LC is suitable for the analysis of compounds with a very wide range of polarities, using experimental variables such as mobile-phase pH and ratio of organic to aqueous mobile phase components to manipulate selectivity and retention to separate sample components from one another.