Reversed Phase Liquid Chromatography
Published Online: 15 SEP 2006
Copyright © 2000 John Wiley & Sons, Ltd. All rights reserved.
Encyclopedia of Analytical Chemistry
How to Cite
Lough, W. J. 2006. Reversed Phase Liquid Chromatography. Encyclopedia of Analytical Chemistry. .
- Published Online: 15 SEP 2006
Reversed-phase liquid chromatography (RPLC) is an elution procedure used in liquid chromatography (LC) in which the mobile phase is significantly more polar than the stationary phase, e.g. a microporous silica-based material with chemically bonded alkyl chains. Typically, the mobile phase is a mixture of an aqueous component, often containing a buffer to control pH, and a water-miscible polar organic solvent. This most frequently used mode of LC is suitable for the analysis of compounds with a very wide range of polarities, using experimental variables such as mobile-phase pH and ratio of organic to aqueous mobile phase components to manipulate selectivity and retention to separate sample components from one another.