Two-Dimensional Nuclear Magnetic Resonance of Small Molecules
Nuclear Magnetic Resonance and Electron Spin Resonance Spectroscopy
Published Online: 15 SEP 2006
Copyright © 2000 John Wiley & Sons, Ltd. All rights reserved.
Encyclopedia of Analytical Chemistry
How to Cite
Pedersen, H. 2006. Two-Dimensional Nuclear Magnetic Resonance of Small Molecules. Encyclopedia of Analytical Chemistry. .
- Published Online: 15 SEP 2006
The article deals with practical aspects in obtaining two-dimensional (2-D) nuclear magnetic resonance (NMR) spectra of small molecules [molecular weight (MW) < 1000]. This includes the choice of parameters for acquisition and processing and the required and optional instrument hardware. Examples of 2-D experiments are given with emphases on robustness and ease of use. The pulse sequences are given in schematic form together with suggestions for basic parameters and the phase cycle. The experiment examples include homonuclear and heteronuclear experiments with direct and indirect detection. Also the use of pulsed field gradients (PFGs) is exemplified. 2-D NMR on spin- nuclei other than 1H and 13C is exemplified by 19F and 15N experiments. Experiments on gel-phase samples under magic angle conditions are mentioned briefly.