Published Online: 15 MAR 2010
Copyright © 2000 John Wiley & Sons, Ltd. All rights reserved.
Encyclopedia of Analytical Chemistry
How to Cite
Schuck, P., Balbo, A., Brown, P. H. and Zhao, H. 2010. Analytical Ultracentrifugation. Encyclopedia of Analytical Chemistry. .
- Published Online: 15 MAR 2010
Analytical ultracentrifugation is a powerful and very versatile technique for the study of particles in solution ranging from sub-nanometers to micrometers in size. It allows the characterization of their size or size distribution, low-resolution (hydrodynamic) shape, density, and solvation properties, and, in some cases, composition and attractive or repulsive interactions. For single- or multicomponent systems with reversible chemical reactions, the number and stoichiometry of complexes, the timescale of their interconversion, as well as binding constants from nanomolar to millimolar range can be determined. Analytical ultracentrifugation is based on first principles and has a long history of theoretical and practical development. This present article provides an introduction of the theoretical concept, the instrumentation, and considerations for conducting experiments and data analysis.