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Dynamic Light Scattering for Protein Characterization

Biomolecules Analysis

  1. Roland Schmidt

Published Online: 15 DEC 2010

DOI: 10.1002/9780470027318.a9092

Encyclopedia of Analytical Chemistry

Encyclopedia of Analytical Chemistry

How to Cite

Schmidt, R. 2010. Dynamic Light Scattering for Protein Characterization. Encyclopedia of Analytical Chemistry. .

Author Information

  1. Manufacturing Science and Technology, Abbott GmbH & Co. KG, Ludwigshafen, Germany

Publication History

  1. Published Online: 15 DEC 2010

Abstract

Dynamic light scattering (DLS) is an analytical tool for the determination of diffusion coefficients and hydrodynamic sizes of molecules and particles in solution. It allows for the characterization in a size range from the nanometer to lower micrometer scale, which makes it especially valuable in the field of biotechnology. The present article provides an introduction to the theoretical concepts of the DLS technique with emphasis on concepts relevant for protein characterization. Applications for protein analysis are discussed and literature references given. Applications include aggregation detection, crystallization monitoring, and association studies. Specialized applications for particle detection at low scattering angles for protein samples, high-throughput analysis, and electrophoretic light scattering (ELS) are highlighted.

Keywords:

  • dynamic light scattering;
  • electrophoretic light scattering;
  • protein;
  • antibody;
  • immunoglobin G (IgG);
  • aggregation;
  • crystallization;
  • binding;
  • association;
  • high concentration;
  • particle detection;
  • physical stress