Dynamic Light Scattering for Protein Characterization
Published Online: 15 DEC 2010
Copyright © 2000 John Wiley & Sons, Ltd. All rights reserved.
Encyclopedia of Analytical Chemistry
How to Cite
Schmidt, R. 2010. Dynamic Light Scattering for Protein Characterization. Encyclopedia of Analytical Chemistry. .
- Published Online: 15 DEC 2010
Dynamic light scattering (DLS) is an analytical tool for the determination of diffusion coefficients and hydrodynamic sizes of molecules and particles in solution. It allows for the characterization in a size range from the nanometer to lower micrometer scale, which makes it especially valuable in the field of biotechnology. The present article provides an introduction to the theoretical concepts of the DLS technique with emphasis on concepts relevant for protein characterization. Applications for protein analysis are discussed and literature references given. Applications include aggregation detection, crystallization monitoring, and association studies. Specialized applications for particle detection at low scattering angles for protein samples, high-throughput analysis, and electrophoretic light scattering (ELS) are highlighted.
- dynamic light scattering;
- electrophoretic light scattering;
- immunoglobin G (IgG);
- high concentration;
- particle detection;
- physical stress