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Liquid Chromatography-Infrared and Size Exclusion Chromatography-infrared Analysis for Polymer Characterization

Infrared Spectroscopy

  1. Monika Plass1,
  2. Andreas Albrecht2,
  3. Robert Brüll2

Published Online: 15 JUN 2010

DOI: 10.1002/9780470027318.a9132

Encyclopedia of Analytical Chemistry

Encyclopedia of Analytical Chemistry

How to Cite

Plass, M., Albrecht, A. and Brüll, R. 2010. Liquid Chromatography-Infrared and Size Exclusion Chromatography-infrared Analysis for Polymer Characterization. Encyclopedia of Analytical Chemistry. .

Author Information

  1. 1

    The Dow Deutschland GmbH & Co OHG, Rheinmünster, Germany

  2. 2

    Deutsches Kunststoff Institute, Darmstadt, Germany

Publication History

  1. Published Online: 15 JUN 2010


Fourier transform infrared spectroscopy (FTIR) can be used as a detector in liquid chromatography, giving detailed information on the molecular structure of the effluents after separation. Achieving structural details of chemical compounds after their separation makes the coupling of interactive liquid chromatography (IC) and size exclusion chromatography (SEC) with IR spectroscopy a versatile analytical tool superior to refractive index (RI), ultraviolet (UV), and evaporative light scattering detectors (ELSDs). LC/IR offers an alternative to LC/MS and LC/NMR to study the chemical composition of the eluted compounds. The hyphenation can be realized in two ways, namely, the on flow with an IR flow cell and off-line via solvent elimination. Both approaches are discussed with regard to their advantages and limitations and different solvent-elimination interfaces are compared. The coupling of LC with FTIR using the IR flow cell and the solvent-elimination interfaces is demonstrated on selected samples, including interactive and size exclusion models at ambient and high temperatures.


  • LC;
  • SEC;
  • FTIR;
  • chemical composition analysis;
  • molecular weight distribution;
  • coupled methods;
  • solvent elimination;
  • flow cell