Standard Article

Frontiers in DNA Sequencing: the (R)Evolution of Sequencing Technologies

Nucleic Acids Structure and Mapping

  1. Shirley Horn-Saban,
  2. Daniela Amann-Zalcenstein

Published Online: 15 DEC 2011

DOI: 10.1002/9780470027318.a9183

Encyclopedia of Analytical Chemistry

Encyclopedia of Analytical Chemistry

How to Cite

Horn-Saban, S. and Amann-Zalcenstein, D. 2011. Frontiers in DNA Sequencing: the (R)Evolution of Sequencing Technologies. Encyclopedia of Analytical Chemistry. .

Author Information

  1. The Weizmann Institute of Science, Rehovot, Israel

Publication History

  1. Published Online: 15 DEC 2011


Over the past 30 years, DNA sequencing evolved from a work-intensive, low-throughput, and high-cost endeavor into an affordable, automated and high-throughput technique for biological research. Currently, we are experiencing the advent of DNA sequencing technology platforms based not only on the long-time prevailing biological techniques, but also on multidisciplinary approaches. While current second-generation technologies rely on massively parallel sequencing of clonally amplified DNA molecules, third-generation technologies are based on single-molecule sequencing. A major motivation for the development of new technologies is meeting the challenge to provide sequencing of an entire human genome for less than US$1000; and what seemed almost impossible a decade ago now seems to have become reality. In this review, we give an overview of the currently available sequencing platforms, as well as a preview of promising new technologies that may enter the market in the near future. We discuss the most common biological applications and how high-throughput sequencing (HTS) has changed and will change the understanding of biological systems.