Next-generation DNA Sequencing Technologies
Nucleic Acids Structure and Mapping
Published Online: 15 DEC 2011
Copyright © 2000 John Wiley & Sons, Ltd. All rights reserved.
Encyclopedia of Analytical Chemistry
How to Cite
Turner, D. J. 2011. Next-generation DNA Sequencing Technologies. Encyclopedia of Analytical Chemistry. .
- Published Online: 15 DEC 2011
The sequencing of multiple polynucleotide templates in parallel, termed next-generation sequencing, has enabled us to generate vast quantities of sequence data very rapidly. As a consequence, large-scale sequencing projects can be undertaken that would not have been feasible previously because the expense and time taken would have been prohibitive. Next-generation technologies can be applied to diverse areas ranging from the de novo genome sequencing of mixed bacterial populations to characterization of the human transcriptome.
This article discusses how sequencing technology has evolved over the past four decades, and the impact that this has had on biological research.