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Next-generation DNA Sequencing Technologies

Nucleic Acids Structure and Mapping

  1. Daniel J. Turner

Published Online: 15 DEC 2011

DOI: 10.1002/9780470027318.a9209

Encyclopedia of Analytical Chemistry

Encyclopedia of Analytical Chemistry

How to Cite

Turner, D. J. 2011. Next-generation DNA Sequencing Technologies. Encyclopedia of Analytical Chemistry. .

Author Information

  1. Oxford Nanopore Technologies, Oxford, UK

Publication History

  1. Published Online: 15 DEC 2011


The sequencing of multiple polynucleotide templates in parallel, termed next-generation sequencing, has enabled us to generate vast quantities of sequence data very rapidly. As a consequence, large-scale sequencing projects can be undertaken that would not have been feasible previously because the expense and time taken would have been prohibitive. Next-generation technologies can be applied to diverse areas ranging from the de novo genome sequencing of mixed bacterial populations to characterization of the human transcriptome.

This article discusses how sequencing technology has evolved over the past four decades, and the impact that this has had on biological research.