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Planar Chromatography Using Electroosmotic Flow

Liquid Chromatography

  1. Tadeusz H. Dzido,
  2. Beata Polak,
  3. Adam Chomicki,
  4. Paweł W. Płocharz

Published Online: 15 SEP 2011

DOI: 10.1002/9780470027318.a9224

Encyclopedia of Analytical Chemistry

Encyclopedia of Analytical Chemistry

How to Cite

Dzido, T. H., Polak, B., Chomicki, A. and Płocharz, P. W. 2011. Planar Chromatography Using Electroosmotic Flow. Encyclopedia of Analytical Chemistry. .

Author Information

  1. Medical University of Lublin, Department of Physical Chemistry, Lublin, Poland

Publication History

  1. Published Online: 15 SEP 2011


Application of electroosmotic flow to drive mobile phase in planar chromatography is presented. There are two techniques of this mode: planar electrochromatography (PEC) and pressurized planar electrochromatography (PPEC). In the former technique, three phases (solid, liquid, and gas) are involved in separation process in the later but two phases (solid and liquid). Theoretical backgrounds of the mode are presented and historical overview as well. The techniques are described regarding development of equipment and methodology in respect of operating variables (composition of the mobile phase, pressure exerted on adsorbent layer, mobile phase flow velocity, temperature of separating system, mode of sample application, etc.) influencing separation efficiency. Advantages of the techniques, especially PPEC, such as high kinetic performance, short time of electrochromatogram development, and different separation selectivity in comparison to conventional thin-layer chromatography (TLC) are presented and discussed. Kinetic performance of PPEC and high-performance liquid chromatography (HPLC) is compared. Two-dimensional separations (2D) using PPEC and TLC are demonstrated to show high separation potential of the mode. The PPEC technique is still in beginning stage of development, so its challenges are discussed as well.