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Biomedical Applications of Raman Spectroscopy

Raman Spectroscopy

  1. Hidetoshi Sato,
  2. Yasuhiro Maeda,
  3. Mika Ishigaki,
  4. Bibin B. Andriana

Published Online: 9 JAN 2014

DOI: 10.1002/9780470027318.a9281

Encyclopedia of Analytical Chemistry

Encyclopedia of Analytical Chemistry

How to Cite

Sato, H., Maeda, Y., Ishigaki, M. and Andriana, B. B. 2014. Biomedical Applications of Raman Spectroscopy. Encyclopedia of Analytical Chemistry. 1–12.

Author Information

  1. Kwansei Gakuin University, Nishinomiya, Japan

Publication History

  1. Published Online: 9 JAN 2014


Raman spectroscopy has specific features suitable for biomedical applications. As it uses visible and near-infrared (NIR) lights, it is possible to use optical fibers to fabricate a stable, robust Raman system and narrow Raman probes. No labeling and no sample preparation are required for spectral measurement and imaging. The broad strong band due to water is observed only in the high-frequency region but not in the fingerprint region. Multivariate analysis is a powerful tool for analyzing the complex spectra of biological samples. Raman spectroscopy is, however, not without drawbacks. It is employed as one of the available modalities in recent biomedical application studies. It may be important to understand its advantages and disadvantages when planning a new study. It is also important to know the feasibility of the desired experiment using the available optics and devices. This article describes the instrumentation, principle, and recent applications, providing an introduction to the research field of biomedical Raman spectroscopy.